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A Broad Spectrum Tissue Culture Experiment with Tobacco ( Nicotiana tabacum ) Pith Tissue Callus
Author(s) -
FOSSARD R. A.,
MYINT AUNG,
LEE EDWARD C. M.
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03116.x
Subject(s) - callus , pith , sucrose , tissue culture , auxin , nicotiana tabacum , botany , biology , chemistry , horticulture , food science , biochemistry , in vitro , gene
The composition and rationale of a broad spectrum tissue culture experiment involving 81 different media are described. Tobacco calluses sub‐cultured on media in this experiment were induced to form six main types of callus depending on the concentration levels of minerals, auxins, cytokinins, and sucrose, growth factors and amino acids in the medium. Only nine of the 81 media inhibited callus growth, and growth (increase in mass divided by initial mass of fresh matter) varied considerably reaching a maximum of nearly 200 after eight weeks on a medium high in minerals, cytokinins, sucrose, growth factors and amino acids and low in auxins. Five media induced regeneration from the calluses. This experiment is suggested as a potentially fruitful introductory test for many new or unresolved tissue culture situations.