Premium
Changes in Endogenous Growth Kegulators in Loblolly Pine Seeds During the Process of Stratification and Germination
Author(s) -
PAUL K. B.,
PATEL C. S.,
BISWAS P. K.
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb08601.x
Subject(s) - germination , stratification (seeds) , loblolly pine , abscisic acid , gibberellin , auxin , biology , endogeny , horticulture , botany , dormancy , seed dormancy , biochemistry , pinus <genus> , gene
The seeds of loblolly pine ( Pirns taeda L.) were cold‐stratified for 0, 14, 28, 42, and 56 days. Endogenous growth regulators were extracted from these seeds, and also from the germinating and the 28‐day warm stratified seeds. Partially purified extracts were separated on chromatographic paper. The chromatograms were cut into 10 equal segments, and these were tested for biological activity using three different btoassays. The results indicated that the unstratified seeds and those stratified up to a period of 28 days contained very little or no growth promoter (GA‐like substances), and a relatively high concentration of an inhibitor (presumably abscisic acid), Following 42‐day stratification, the promoter concentration gradually increased while the inhibitor level fell almost to zero. A high level of promoter but no inhibitor was detected in germinating seeds. No auxin‐like activity was noted in the unstratified seeds. This activity slowly increased up to a period of 28 days and remained at this level for the subsequent stratification periods. However, the activity greatly increased in the germinating seeds. Very little changes in the levels of growth regulators were noted in warm‐stratified seeds as compared to the unstrati‐fied controls.