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The Intra‐cellular Localization of the Herbicide Diphenamid in Corn Root .Tip
Author(s) -
YAKLICH R. W.,
SCOTT E. G.
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb08587.x
Subject(s) - differential centrifugation , microsome , elution , centrifugation , zea mays , chemistry , chromatography , pesticide , size exclusion chromatography , cell wall , biology , botany , agronomy , biochemistry , enzyme
The intra‐cellalar localization of the herbicide N,N‐dimethyl–2,2‐diphenylacetamide (diphenamid) was examined in 10 mm root tips of corn seedlings ( Zea mays L. cv. Shawnee). Experiments are described which culminate in the observation that a significant, measurable effect on root growth occurs within 12 hours after treatment with diphenamid. Corn seedlings were then treated with 14‐C‐diphenamid for 12 hours and the 10 mm root tips excised and homogenized. By use of differential centrifugation and gel filtration, the root homogenate was separated into fractions; as follows; cell wall, mitochondria, microsomal, protein and eluate. The highest concentration of the herbicide was found in the root tips. Furthermore, most of the radioactivity was found in the eluate. Results are discussed as to localization of diphenamid in the microsomal and protein fraction and possible mode of action.