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A Technique for Studying the Adsorption, Absorption and Metabolism of Amino Acids in Intact Apple Stem Tissue
Author(s) -
HILLCOTTINGHAM D. G.,
LLOYDJONES C. P.
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb08586.x
Subject(s) - amino acid , xylem , chemistry , absorption (acoustics) , adsorption , aqueous solution , salt (chemistry) , metabolism , biochemistry , chromatography , botany , organic chemistry , biology , materials science , composite material
Solutions of amino acids are introduced rapidly into the xylem of short lengths of stem. At sampling the intact stem is first perfused with a salt solution to remove the amino acids still reversibly adsorbed by cation exchange. This fraction of the applied amino acids is thus distinguished from that absorbed into the tissues which is subsequently extracted from the disintegrated stem by aqueous ethanol. The potential value of this technique is discussed and illustrated with some results using carbon‐14 labelled amino acids in apple stems.