Premium
Ozone Induced Carbon Dioxide Evolution in Tobacco Callus Cultures
Author(s) -
ANDERSON WILBUR C.,
TAYLOR O. C.
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb08582.x
Subject(s) - ozone , callus , carbon dioxide , chemistry , botany , nutrient , horticulture , environmental chemistry , biology , organic chemistry
Callus derived from Bel–W3 and Bel–B tobacco plants when exposed to ozone turned brown as a consequence of surface cell destruction. Ozone fumigations above a threshold concentration of 0.10 μl/1 for two hoars caused an increase in the rate of tissue carbon dioxide (CO 2 ) evolution. The maximum increase in CO 2 evolution was about 65 percent for both the ozone sensitive Bel–W3 and resistant Bel–B callus. However, the ozone dosage required to attain maximum increase in CO 2 evolution was approximately two times greater for the resistant variety. Callus cultures that grew roots were observed to be more resistant to ozone. The addition of the antioxidant N,N'dipnenyl–p–phenylenediamine (DPPD) m the nutrient medium retarded ozone induced CO 2 evolution.