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Development of Ascorbate Oxidase Activity and Its Iso‐enzyme Pattern in the Roots of Pea Seedlings
Author(s) -
SUZUKI YONEZO,
OGISO KAZUKO
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb03086.x
Subject(s) - epicotyl , germination , seedling , enzyme , chemistry , enzyme assay , oxidase test , biochemistry , biology , horticulture
Early in the germination of peas, ascorbate Oxidase activity increased markedly in the root and epicotyl, but not in the cotyledons. The activity in the root was suppressed by the administration of 6‐methylpurine, thiouracil, cyclohexi‐mide, thioproline, and phenylthiourea. Although enzyme development was inhibited by phenylthiourea and thioproline, root elongation was not affected by these reagents. As germination progressed, the ascorbate oxidase activity in the extract of the seedling's root could be separated into at least 3 to 7 isoenzymes.