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Carbon Dioxide and pH Requirements of Non‐Photosynthetic Tissue Culture Cells
Author(s) -
NESIUS KNEELAND K.,
FLETCHER JOHN S.
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb01186.x
Subject(s) - carbon dioxide , carbon fixation , phosphoenolpyruvate carboxylase , photosynthesis , dry weight , pyruvate carboxylase , suspension culture , zoology , chemistry , biology , botany , biochemistry , ecology , cell culture , enzyme , genetics
Maximum growth of suspension cultures of Paul's Scarlet rose required a low pH (5.2 to 5.4) during the division phase (day 0 to 7) and a higher pH (5.8 to 6.0) during the expansion phase (day 7 to 14). The fresh weight increase was reduced by approximately 22%, but the dry weight was not influenced when cells were grown for 14 days in a CO 2 deficient environment. Kinetic studies showed that the first five days of growth was the critical period of nonautotrophic CO 2 fixation when cells were grown in medium buffered at pH 5.4. The phosphoenolpyruvate carboxylase activity was highest (0.50 × 10 6 cpm min −1 · g −1 fresh weight) during the period when nonautotrophic CO 2 fixation appeared to be critical for growth.