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Protoplast Cultures from Cell Suspensions of Daucus carota
Author(s) -
WALLIN ANITA,
ERIKSSON TAGE
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb01147.x
Subject(s) - daucus carota , protoplast , petri dish , sucrose , biology , sorbitol , agar , botany , cell wall , suspension culture , biochemistry , cell culture , microbiology and biotechnology , bacteria , genetics
Protoplasts, enzymatically isolated from cell suspension cultures of Daucus carota , have been grown in small Petri‐dishes. After enzyme treatment and washing the protoplasts were plated in agar media. Growth and divisions were viewed through the bottom of the Petri‐dishes with a light microscope. Different osmotic stabilizers were tested with respect to their ability to promote wall formation and growth of the protoplasts. Combinations of sucrose, sorbitol and “Modopeg” gave the best results. Electron micrographs of cultured protoplasts revealed normal as well as abnormal nuclear conditions.