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Starch Metabolism, Respiration, and Shoot Formation in Tobacco Callus Cultures
Author(s) -
THORPE TREVOR A.,
MEIER DONALD D.
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03629.x
Subject(s) - callus , shoot , starch , respiration , tissue culture , botany , gibberellic acid , biology , darkness , metabolism , respiration rate , chemistry , biochemistry , in vitro , germination
The starch content of shoot‐forming and non‐shoot‐forming tobacco callus cultured in light and darkness was determined. A variety of carbohydrates and cytokinins incorporated into the culture medium were effective in bringing about starch accumulation and shoot formation in the tissue. In addition, the respiratory activity of the callus, grown in the presence or absence of gibberellic acid, was measured. A strong correlation between the starch content of the tissue, its rate of respiration, and shoot formation was observed.