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The Fate of Exogenously Applied Indoleacetic Acid in Light Grown Stems
Author(s) -
DAVIES P. J.
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03612.x
Subject(s) - pisum , sativum , glucoside , chemistry , incubation , ethanol , chromatography , biology , biochemistry , botany , alternative medicine , pathology , medicine
Elongating segments from light grown pea ( Pisum sativum L. cv. Alaska) and bean ( Pbaseolus vulgaris L. cv. Red kidney) stems were incubated in 10 ‐5 M indoleacetic acid (IAA)‐1‐ 14 C,and ‐5‐ 3 H in the light. Radoactive derivatives, extracted in water, ethanol or ether, and 1 N sodium hydroxide at three different times were chromatographed in three separate systems and the different metabolites identified by their labeling and chromatographic characteristics. Major metabolites included indoleacetyl aspartate, possibly indoleacetyl glucoside, hydroxymethyloxindole, and in bean a further major unidentified compund. Other compounds occurred in lesser amounts. Indole aldehyde was present only in very small quantities. IAA breakdown commenced between 1 and 6 h of incubation, following which IAA decreased and most metabolites increased, though IAA was still present after 24 h. Alkaline hydrolysates contained mainly IAA at a level which changed little between 6 and 24 h.