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Effect of Temperature and Some Antimetabolites on the Interaction Effects of Auxin and Nutrition in Rooting Etiolated Stem Segments of Salix tetrasperma
Author(s) -
JAIN M. K.,
NANDA K. K.
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03595.x
Subject(s) - auxin , cycloheximide , primordium , etiolation , biology , botany , biochemistry , enzyme , protein biosynthesis , gene
Rooting on 2.5 cm long stem segments of Salix tetrasperma was optimal when cultured in 5.0 mg/I IAA in combination with 1.0 % glucose at 30°C, and in combination with 0.1 % glucose at 15°C. 5‐Fluorodeoxyuridine (FUDR), actinomycin‐D and cycloheximide all inhibited rooting and the inhibitory effect in each case increased with concentration. 5‐Fluorouracil (FU), on the other hand, was ineffective. The results show that auxins probably act as a triggering agent for the synthesis of specific enzyme proteins that are required for the initiation of root primordia at the transcriptional level and that a correct balance of auxin and nutrition which may vary with the environmental conditions, is necessary for optimal rooting.