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Scanning Electron Microscopy of the Nematode‐trapping Organs in Arthrobotrys oligospora
Author(s) -
NORDBRINGHERTZ BIRGIT
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03581.x
Subject(s) - scanning electron microscope , fungus , mycelium , agar , nematode , electron microscope , materials science , biology , botany , composite material , optics , ecology , genetics , physics , bacteria
The three‐dimensional capture organs of the nematodetrapping fungus Arthrobotrys oligospora are presented in scanning electron micrographs. The fungus was grown directly on agar substrates or on millipore filters or glass cover slips placed on agar substrates. To avoid shrinkage of the soft‐walled mycelial material, a fixation in O s O 4 ‐vapour was performed, before freeze‐drying and coating with Au‐Pd alloy. When capture organs were prepared in this way, it was possible to visualize them in their erect position in the scanning electron microscope. Young capture organs, both spontaneously formed and nematode‐induced, remained standing better than old ones. An adhesive substance on the capture organs, with the aid of which nematodes are trapped, seems to be visible in these pictures.