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Changes in Nicotinamide Nucleotide Coenzymes in Cucumber Leaves during Ammonium Toxicity
Author(s) -
WAKIUCHI N.,
MATSUMOTO H.,
KONDO S.,
TAKAHASHI E.
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03573.x
Subject(s) - nad+ kinase , cofactor , nicotinamide , cucumis , ammonium , nucleotide , biochemistry , ammonium acetate , toxicity , chemistry , biology , enzyme , botany , chromatography , organic chemistry , gene , high performance liquid chromatography
Nicotinamide nucleotide coenzymes were estimated enzymatically in cucumber leaves ( Cucumis sativus L. cv. Suisei No. 2) during ammonium toxicity. The contents of all the coenzymes (NAD(H) and NADP(H)) were found to be higher in the ammonium‐treated plants than in the control plants, and the difference attained a maximum at 5 days after the initiation of ammonium treatment. Thereafter, the contents of NAD and NADH returned towards the control level, but NADP and NADPH levels were lowered in injured plants. The ratios of NAD/NAD + NADH and NADP/NADP ++ NADPH were little altered by the ammonium treatment. Changes of nicotinamide nucleotide coenzymes are discussed in relation to respiratory metabolism in cucumber leaves during ammonium toxicity.