Premium
Interaction of Kinetin and Indoleacetic Acid in the Avena Straight‐Growth Test
Author(s) -
HEMBERG TORSTEN,
LARSSON ULF
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03552.x
Subject(s) - kinetin , avena , auxin , coleoptile , chemistry , chromatography , stimulation , botany , biochemistry , biology , in vitro , tissue culture , neuroscience , gene
Kinetin has a stimulating effect in the Avena straight‐growth test. The action of different concentrations of kinetin, 2.5 × 10 −7 , 2.5 × 10 −6 and 2.5 × 10 −5 M , in combination with different concentrations of IAA was studied in this test. It was shown that the effect of low IAA concentrations, 0.25 × 10 −7 and 1 × 10 −7 M , was strongly enhanced by the addition of all the kinetin concentrations investigated. The effect of the highest IAA concentrations, 25 × 10 −7 and 100 × 10 −7 M , on the other hand, was inhibited relatively strongly by the highest employed concentration of kinetin. The results are explained as due to a kinetin‐produced increase of auxin in the coleoptile segment, which in combination with low IAA concentrations can lead to a growth stimulation and with high IAA concentrations to a growth inhibition. Since kinetin in purification and chromatography of auxin can partly follow IAA, thereby affecting the quantitative yield, it is emphasized that, prior to the test, auxin extracts containing cytokinins should be freed from the latter by, for example, gel filtration or paper electrophoresis.