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Deoxyribonucleotide Pools in Plant Tissue Cultures
Author(s) -
NYGAARD PER
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03541.x
Subject(s) - deoxyribonucleoside , ribonucleoside , callus , nucleoside , perchloric acid , biochemistry , chemistry , deoxyribonucleotide , nucleoside triphosphate , tissue culture , chromatography , nucleotide , biology , dna , botany , rna , organic chemistry , in vitro , gene
Two dimensional thin‐layer chromatography on anion‐exchange cellulose enables the separation of the normally occurring ribo‐ and deoxyribonucleoside triphosphates. This technique was applied to perchloric acid extracts of callus tissue of sycamore and tobacco and of pine pollen grown in 32 P‐orthophosphate labelled media to quantitate the nucleoside triphosphate pools under different growth conditions. The results showed that the ratio of the deoxyribonucleo‐side triphosphates to their corresponding ribonucleoside triphosphates is low in plant cells, similar to the ratio previously found for animal cells. During the period of most rapid DNA synthesis in the callus tissue, the deoxyribonucleoside triphosphate pools reach their highest values. This effect is also demonstrated with cells of Escbericbia coli .