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Wall‐bound Invertase Activity in Convolvulus Callus: Increase after Subculturing, and Paradoxical Effects of Actinomycin D, Cycloheximide, and Thienylalanine
Author(s) -
KLIS F. M.,
HAK A.
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb01123.x
Subject(s) - invertase , cycloheximide , callus , cell wall , cytoplasm , chemistry , biochemistry , subculture (biology) , enzyme , botany , biology , protein biosynthesis
The wall‐bound invertase activity increased 3.3‐fold upon transfer of fragments of Convolvulus callus to fresh solid nutrient medium and 7.7‐fold upon transfer to liquid nutrient medium. Addition of actinomycin D, cycloheximide or the amino acid analogue thienylalanine brought about a further stimulation of the invertase content of the cell walls. The rise of wall‐bound invertase activity was not due to redistribution of invertase activity between cytoplasm and cell walls, and appeared to be dependent on metabolic energy. An equation is presented to calculate the half‐life of enzymes from their time‐course. Applied on the time‐courses of wall‐bound invertase activity, a half‐life of about 12 h was obtained in callus transferred to fresh solid medium and of about 5.4 h in tissue transferred to liquid medium. It is argued that the increase of invertase content of the cell walls is due to an enhanced rate of invertase synthesis.