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Variants of Myxococcus virescens Exhibiting Dispersed Growth. Growth and Production of Extracellular Enzymes and Slime
Author(s) -
HASKÅ GUNO,
STÅHL STEN
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb06731.x
Subject(s) - enzyme , growth medium , polysaccharide , bacteria , proteolytic enzymes , extracellular , biochemistry , bacterial growth , biology , doubling time , chemically defined medium , cell growth , stationary phase , food science , chemistry , cell , chromatography , in vitro , genetics
The growth of two strains of Myxococcus virescens exhibiting dispersed growth was followed in casamino acids (N III‐C) media and casitone media. The changes in optical density, pH, pigmentation as well as the secretion of bacteriolytic and proteolytic enzymes, DNA and polysaccharides during growth were recorded. In both media the bacteria grew exponentially with a generation time of 4 (casitone) and 20 hours (N III‐C) respectively. The maximal cell mass was about 4 times higher in casitone than in casamino acids media. The amounts of bacteriolytic enzymes produced by the two strains in N III‐C medium were different but in casitone medium they were about equal and considerably higher. The maximal values of proteolytic enzymes were about the same in both media and always occurred later than the bacteriolytic maxima. Both activity peaks appeared before the phase of decline. The polysaccharide production reached a maximum during the stationary growth phase in both media. A higher value was reached during growth in casitone medium than in N III‐C medium. During the phase of decline a second increase of polysaccharide in the medium appeared. No DNA could be detected in the cell‐free solutions until the beginning of the phase of decline.