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Autoradiographic Studies of Pear Pollen Tube Walls
Author(s) -
ROGGEN H. P.,
STANLEY R. G.
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb06721.x
Subject(s) - pollen tube , callose , pectinase , pollen , pear , cellulose , cell wall , botany , tube (container) , germination , cytoplasm , pectin , biophysics , biology , chemistry , biochemistry , materials science , enzyme , composite material , pollination
Germinating pollen was supplied D‐glucose‐ 3 H and myo‐inositol‐ 3 H. Pollen tube growth was terminated at varying intervals and the cytoplasmic contents removed or left intact. Empty pollen tubes were treated with β‐1,4‐glucanase, β‐1,3‐glucanasc or pectinase and the residual radioactivity was determined. Analysis of autoradiograms showed the pollen tip contained primarily pectin, the subtending cell wall of the tube was primarily cellulose, or possibly hemi‐cellulose, and callose accumulated most heavily toward the rear, older segments of the tube. Callose was also heavily formed in the tip region of non‐growing tubes. Pectin formed from myoinositol appeared to be solubilized, or removed, from the wall to the cytoplasm with aging of the pollen tube.