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Role of Cell Wall‐degrading Enzymes in Auxin‐induced Cell Expansion in Yeast
Author(s) -
SHIMODA CHIKASHI,
YANAGISHIMA NAOHIKO
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb06713.x
Subject(s) - yeast , auxin , cell wall , saccharomyces cerevisiae , enzyme , biochemistry , cell , biology , chemistry , microbiology and biotechnology , gene
Using auxin‐responsive diploid strains of Saccharomyces cerevisiae and S. ellipsoideus , we studied the role of cell wall‐degrading enzymes in the auxin‐induced cell expansion. Highly purified fungal β‐l,3‐glucanase added to cell suspension caused cell expansion in these strains. The cell expansion induced by auxin was inhibited by the addition of õ‐glucono‐lactone, which inhibited the activity of a crude β‐l,3‐gluca‐nase preparation from yeast in a competitive manner. Laminarinase activity was significantly higher in the extract from auxin‐treated yeast cells than in the extract of cells not treated with auxin. These results support the idea that auxin induces expansion of yeast cells of certain strains through enhancement of the activity of wall polysaccharide‐degrading enzymes.