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Regulation of Growth in Avena Stem Segments by Gibberellic Acid and Kinetin
Author(s) -
JONES R. A.,
KAUFMAN P. B.
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb03524.x
Subject(s) - kinetin , avena , meristem , gibberellic acid , gibberellin , biology , cytokinin , cell division , botany , auxin , biochemistry , cell , in vitro , tissue culture , germination , shoot , gene
Kinetin at physiological concentrations causes significant reduction of GA 3 ‐promoted growth in excised Avena stem segments. Kinetin is therefore considered to be a gibberellin‐antagonist in this system. A Lineweaver‐Burke plot reveals that kinetin acts non‐competitively with GA 3 . The kinetin inhibition of GA 3 ‐promoted growth can be seen within 6 hours. It was found that soluble protein is markedly increased by kinetin in the tissue during the first 3 hours, thus preceding the inhibition of GA 3 ‐promoted growth by several hours. At the cellular level, kinetin negated the blocking effect of GA 3 on cell division in the intercalary meristem portions of these segments. In fact, kinetin promotes both lateral and longitudinal cell divisions in intercalary meristem cells.