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Decarboxylation and Transport of Auxin in Segments of Sunflower and Cabbage Roots. II. A Chromatographic Study Using IAA‐1‐ 14 C and IAA‐5‐ 3 H
Author(s) -
AASHEIM TORBJÖRN,
IVERSEN TORHENNING
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb03498.x
Subject(s) - auxin , helianthus , chemistry , sunflower , reagent , brassica , brassica oleracea , helianthus annuus , chromatography , botany , horticulture , biology , biochemistry , gene
The polar movement of IAA has been examined in 5‐mm root segments of Brassica oleracea and Helianthus annum . The movement was studied partly with IAA‐1‐ 14 C and partly with IAA‐5‐ 3 H. In both plants a slight acropetal flux of 14 C and IAA‐ 3 H was found through the segments. The recovered radioactivity in the agar receiver blocks and in the receiver end of the segments increased as a function of time. A large portion of the applied IAA was converted on the cut surfaces and in the tissues of the segments. Chromatographic analysis indicated different destruction products when estimated by scintillation counting and by spraying with in‐dole reagent (DMCA). Chromatograms run in isopropanol: ammonia: water, 8:1:1, yielded three different substances, one spot near the starting line and one near the front, neither of which has been identified. Finally there was a spot with R f 0.4–0.6, probably representing IAA.