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Quantitative Relationship between Various Growth Substances and Bud Production in Funaria hygrometrica . A bioassay for abscisic acid
Author(s) -
VALADON L. R. G.,
MUMMERY ROSEMARY S.
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb03484.x
Subject(s) - abscisic acid , kinetin , bioassay , gibberellic acid , cytokinin , biology , botany , biochemistry , tissue culture , germination , in vitro , auxin , ecology , gene
Eleven growth substances were tested for their bud producing capacity in Funaria hygrometrica . IAA and gibberellic acid showed no response, neither did inositol, abscisic acid and adenine. The cytokinins: benzylaminopurine, kinetin and mercaptopurine showed a quantitative relationship while methylaminopurine did not. When different amounts of abscisic acid were added together with 10 μg kinetin (which gave maximal production of buds), there existed a linear relationship between reduction in buds and abscisic acid added. Results are obtained in two weeks and it is therefore a fairly quick method for assaying certain cytokinin and also abscisic acid.