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The Effect of Reaction Media on the Coupling and Uncoupling of Respiration in Corn Mitochondria
Author(s) -
KOEPPE D. E.,
MILLER RAYMOND J.
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb03475.x
Subject(s) - bovine serum albumin , respiration , chemistry , mitochondrion , sucrose , biochemistry , calcium , gramicidin , dinitrophenol , chromatography , biology , membrane , organic chemistry , botany
The coupling and uncoupling properties of isolated corn mitochondria were analyzed using three substrates in Tris buffered sucrose and KC1 reaction medias containing inorganic phosphate (P i ), bovine serum albumin (BSA), or P i and BSA. In these media, without other cofactors, respiratory control (RCR) and ADP/O ratios, and the respiratory burst affected by dinitrophenol (DNP), gramicidin D, calcium chloride and ADP were measured. Bovine serum albumin enhanced the respiratory burst caused by DNP and gramicidin D in the absence of P i , and in most instances enhanced the stimulation of oxygen uptake by ADP and calcium chloride in the presence of P i . Mitochondria oxidizing succinate, malate‐pyruvate or NADH exhibited better RCR and ADP/O ratios in buffered 200 m M KCl than they did in buffered 300 m M sucrose. In all instances RCR and ADP/O ratios were enhanced in reaction medias containing BSA.