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The Effect of Growth Regulators and Cu 2+ on the Activation of Amylopectin‐l,6‐glucosidase Activity in Pea Seedlings
Author(s) -
VLODAWSKY LEA,
HAREL E.,
MAYER A. M.
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb01456.x
Subject(s) - amylopectin , zymogen , enzyme , biochemistry , proteolytic enzymes , chemistry , enzyme assay , liberation , germination , biology , in vitro , botany , amylose , starch
No evidence could be obtained for hormonal control of amylopectin‐l,6‐glucosidase activity in germinating peas for the first 72 hours of germination. The embryonic axis did not stimulate the appearance of enzyme activity. The autolytic system which releases amylopectin‐l,6‐glucosidase activity from the particulate fraction, in which it originates, was studied in greater detail. Using Cu 2+ ions to inhibit a proteolytic enzyme in vivo , it was shown that enzyme activation can occur in the zymogen‐like granules without liberation of the enzyme into the soluble cell fraction. Activity so formed is labile. Some of the data on proteolytic enzymes in peas is discussed and an attempt made to interpret the liberation of amylopectin‐l,6‐glucosidase in peas on the basis of the involvement of at least two distinct proteolytic enzymes.