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Effect of α‐Naphthalene‐ and α‐Naphthoxyacetic Acid on the Activity of Certain Enzymes of Atropa belladonna cv. lutea Cells in Suspension Culture
Author(s) -
SIMOLA LIISA KAARINA,
SOPANEN TUOMAS
Publication year - 1971
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1971.tb01079.x
Subject(s) - biochemistry , enzyme , pectinase , enzyme assay , atropa belladonna , gibberellic acid , biology , transaminase , botany , germination
The growth of Atropa belladonna cv. lutea Döll cells and the activities of several enzymes were studied as a function of time in suspension cultures without added auxin, with α‐naphthaleneacedc acid (NAA) and with α‐naphthoxyacetic acid (NOA). The growth curves were rather similar and therefore easy to compare. The enzyme activities (per mg dry weight of culture) were distinctly higher in cultures grown with NAA than in the other two treatments, in which root formation was common. In some cases the enzyme activities were lower in cultures with NOA than in the control series but usually the curves were quite parallel. Promotion of enzyme activities by NAA (aldolase, aminopeptidase, gluta‐mate: oxaloacetate transaminase) was clearest in the beginning of the phase of rapid cell division (6‐day‐old culture). These three enzymes reach their maxima at this stage of growth in the other series as well. Ribonuclease activity increased in the stationary phase cells (13 and 19 days) with NAA but with the other treatments the level of this activity was relatively stable. Peroxidase and phosphatase did not show any characteristic maxima, glutamate: pyruvate transaminase activity was low and no amylase activity could be demonstrated. The results indicate that synthetic plant growth substances can regulate the physiological state of a tissue by causing alterations in enzyme activities, but their action is clearly specific.