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Effects of Abscisic Acid on Phenolic Content and Lignin Biosynthesis in Tobacco Tissue Culture
Author(s) -
Li H. C.,
Rice E. L.,
Rohrbaugh L. M.,
Wender S. H.
Publication year - 1970
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1970.tb06490.x
Subject(s) - scopoletin , callus , chlorogenic acid , abscisic acid , chemistry , lignin , biochemistry , tissue culture , botany , food science , organic chemistry , biology , in vitro , medicine , alternative medicine , pathology , gene
A significant depression of callus growth resulted from low concentrations of abscisic acid (ABA) added to the medium recommended by Linsmaier and Skoog. Low concentrations also decreased the chlorogenic acid and lignin content of the callus, and generally decreased amounts of scopolin and scopoletin in the tissue. Gibberellic acid (GA 3 ) stimulated callus growth in a low concentration (0.1 mg/1) and inhibited growth at a high concentration (10.0 mg/1). Both levels of GA 3 increased scopoletin accumulation in tobacco callus. A high concentration of GA 3 increased the accumulation of scopolin and chlorogenic acids, whereas a low concentration decreased the amounts of these two phenolic compounds. In comparison with the control, lignin synthesis was stimulated by a low GA 3 concentration, but a high GA 3 concentration did not have a significant effect. Both low and high concentrations of GA 3 overcame ABA inhibition of growth and lignin synthesis, and partially reversed ABA inhibition of scopoletin production. However, GA 3 did not reverse the inhibitory effect of ABA on scopolin production. The low concentration of GA 3 overcame the inhibition of chlorogenic acid production resulting from a 0.01 mg/1 concentration of ABA, but this was the only reversal of chlorogenic acid inhibition resulting from addition of GA 3 to the medium.