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Effect of Fluoride on Ribosomes from Corn Roots. Changes with Growth Retardation
Author(s) -
Chang Chong W.
Publication year - 1970
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1970.tb06445.x
Subject(s) - ribosome , ribosomal rna , rna , ribosomal protein , fluoride , centrifugation , eukaryotic ribosome , differential centrifugation , protein biosynthesis , chemistry , moiety , biochemistry , biology , chromatography , stereochemistry , inorganic chemistry , gene
Growth inhibition caused by fluoride was further studied by analyzing free and bound ribosomes, and the ribosomal components in corn roots. Ribosomes were isolated by differential centrifugation. Ribosomal components were analyzed by column chromatography and electrophoresis. Fluoride reduces the amounts of both free and bound ribosomes. Fluoride also modifies the RNA to protein ratio of bound ribosomes more than that of free ribosomes. Fluoride does not affect base ratios of the ribosomal RNA, but reduces its content and changes the structure of the ribosomal protein moiety.