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The Role of Ethylene Diisothiocyanate (EDI) in the Antifungal Action of Disodium Ethylene Bisdithiocarbamate (Nabam). I. The Mode of Action of EDI on the Metabolism of Yeast
Author(s) -
Håbrekke J. I.,
Goksøyr J.
Publication year - 1970
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1970.tb06443.x
Subject(s) - ethylene , respiration , yeast , flavoprotein , biochemistry , chemistry , metabolism , ethanol , carbohydrate metabolism , citric acid cycle , glycolysis , biology , enzyme , catalysis , botany
With glucose as a substrate, the oxygen consumption in yeast in inhibited by 2· 10 ‐5 M ethylene diisothiocyanate. The degree of inhibition was only to a small extent dependant on pH. Radiorespirometric experiments with uniformely labelled glucose showed that the CO 2 ‐production from glucose increased, probably due to increased glycolytic activity. Conversion of C‐1 to CO 2 was unaffected by the inhibitor, while the evolution of CO 2 from C‐6 was strongly inhibited. The same was the case with CO 2 from C‐1 in acetate. Respiration of ethanol was more strongly inhibited than that of glucose or acetate. Experiments with dual wavelength spectrophotometry showed the inhibition to be located on the Krebs cycle side of the respiratory flavoproteins. It is concluded that the action of ethylene diisothiocyanate on respiration must be located at the mitochondria.