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Auxin‐induced Growth of Helianthus Tuber Tissue V. Role of DNA synthesis in hormonal control of cell growth
Author(s) -
Kamisaka Seiichiro,
Masuda Yoshio
Publication year - 1970
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1970.tb06424.x
Subject(s) - auxin , thymidine , dna synthesis , gibberellic acid , biochemistry , gibberellin , dna , cell growth , biology , uridine , ammonium chloride , hormone , chemistry , botany , rna , germination , organic chemistry , gene
The role of DNA synthesis in hormonal control of cell growth was studied, using tissue slices excised from cold‐ stored Helianthus tubers. Metabolic inhibitors of DNA synthesis such as 5‐fluorodeoxyuridine and phenethyl alcohol inhibited the effect of auxin and gibberellie acid on expansion growth, the latter being given during the aging period. The inhibitory effect of 5‐fluorodeoxyuridine on expansion growth was alleviated by the simultaneous addition of thymidine but not by that of uridine. Incorporation of 14 C‐thymidine into DNA occurred during the aging period and gibberellic acid stimulated the incorporation of 14 C‐thymidine into DNA when given during the aging period. Mitomycin C and 2‐(chloroethyl)‐trimethyl‐ammonium chloride (a growth retardant) inhibited incorporation of labelled thymidine into DNA.