Premium
Synthèse des RNA au cours de la germination du radis
Author(s) -
Julien R.,
Grellet F.,
Guitton Y.
Publication year - 1970
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1970.tb06422.x
Subject(s) - rna , ribosomal rna , ribosome , germination , biology , microbiology and biotechnology , chemistry , biochemistry , botany , gene
Abstract RNA synthesis in radish is studied during the first stages of germination. The radish seeds allowed to germinate in the dark, on distilled water, synthesize ribosomal RNA and accumulate a particular RNA, not incorporated in ribosomes. The results of 32 P incorporation in RNA of radish seedlings indicate a progressive formation of ribosomal RNA. Two species of rapidly labelled RNA are synthesized. With labelling time, their chromatographic behaviour on MAK columus evolves, while their electrophoretic characteristics remain stable. It is assumed that these two species are involved in ribosome formation. In vivo experiments with chloramphenicol support this conclusion. RNA which accumulates during germination, could be a particular type of ribosomal RNA which could be enable, under the definite culture conditions, to enter into ribosomal structures.