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Occurrence of Indole‐3‐Acetic Acid in Buds of Pinus silvestris
Author(s) -
Aldén Torsten,
Eliasson Lennart
Publication year - 1970
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1970.tb06402.x
Subject(s) - coleoptile , chemistry , auxin , scots pine , acetic acid , indole 3 acetic acid , pinus <genus> , sephadex , chromatography , botany , ethanol , elution , indole test , avena , biology , stereochemistry , biochemistry , enzyme , gene
The major ether‐soluble, growth‐stimulating substance detected by the Avena coleoptile straight‐growth test in extract from sprouting buds of Scots pine ( Pinus silvestris L.) was identified as indole‐3‐acetic acid by Rf values in 5 solvent systems and by its elution volume in ethanol on a Sephadex LH‐20 column. When the substance was applied to the growth solution of wheat roots in a special test the growth in length of the roots was at first inhibited, but growth was recovered after about 6 hours in the same manner as when small quantities of IAA were applied. The extracts also contained large amounts of growth inhibitors which interfered with the auxin response if they were not removed.