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Succinyl CoA Synthetase of Tobacco
Author(s) -
Bush Lowell P.
Publication year - 1969
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1969.tb09097.x
Subject(s) - nicotiana tabacum , meristem , enzyme , biochemistry , enzyme assay , germination , specific activity , succinic acid , seedling , biology , chemistry , botany , gene
The isouition and partial purification of succinyl CoA synthetase from Nicotiana tabacum is described. Enzynio activity had a pH optimum of pH 7.0 and apparent Km values for succinic acid. ATF and MgCl 2 . are 3.5 × 10 −2 5.7 × 10 −3 and 4.0 × 10 −3 M, respectively. The euzyme is specific in requirement for ATP. Enzyme activity was low in seed, decreased during germination and seedling development, then increased to maximum in physiologically mature leaf tissue. Plauts with the apical apical meristem removed had less euzyme activity in leaves but more enzyme activity in the roots than plants with apical meristem intact. Most enzyme activity observed was associated with the soluble fractions of the cell.