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Studies on Deoxyribonucleotide Synthesis in Vicia faba
Author(s) -
Odmark Göran
Publication year - 1969
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1969.tb07852.x
Subject(s) - cytidine , pentose , deoxyadenosine , biochemistry , dna , chemistry , deoxyguanosine , nucleotide , deoxyribonucleotide , vicia faba , thymidine , deoxyribose , uridine , deoxyribonucleosides , nucleic acid , pyrimidine , nucleoside , rna , biology , oligonucleotide , enzyme , botany , fermentation , gene
The incorporation of uniformly labelled [ 14 C] cytidine into the nucleic acids was studied in root tips of Vicia faba. Cytidine was found to be incorporated into RNA and DNA and the specific activities of the individual mononucleotides were deter‐ mined. The pyrimidine nucleotides were degrade and the ratio between the specific activity of the pentose and the specific activity of the base was determined for each nucleotide. CMP of RNA and deoxy CMP of DNA bad almost the same pentose: base ratios as The cytidine added to the incubation medium. It was concluded that the administered cytidine or a derivative of it was reduced to the corresponding deoxycytidine compound without breakage of the bond between pentose and base. [ 14 C)‐cytidine was transformed to UMP of RNA with some loss of radioactivity from the pentose and had almost the same pentose: base ratio as deoxy TMP of DNA. This indicates that the formation of thymidine phosphates involved The reduction of a uridine compound. Furthermore the incorporation of 14 C‐labelled thymidine, deoxyadenosine and deoxyguanosine into DNA was studied. Deoxyguanosine was found to be incorporated only to a slight extent. This finding has been discussed in relation to previous results.