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The Inhibition of Seedling Growth by Streptomycin Sulfate
Author(s) -
Druhy Robert E.,
Khan Anwar A.
Publication year - 1969
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1969.tb07432.x
Subject(s) - mitosis , cell division , biology , shoot , seedling , nucleic acid , biochemistry , germination , rnase p , dna synthesis , cell growth , growth inhibition , mitotic index , in vitro , botany , cell , microbiology and biotechnology , rna , gene
The action of streptomycin sulfate (SS) on the growth of germinating seeds was investigated. It inhibited root growth more than shoot growth. Chemical analysis of roots indicated that nucleic acids decreased but their elution pattern from a column of methylated albumin kieselghur showed no qualitative differences. SS decreased the number of cells undergoing mitosis per root tip. This number was correlated with root length. One nM N 5 ‐benzyladenine reversed the effect of SS on both root length and mitosis. Thus the effect of SS appears specific to cell division. SS failed to affect oxidative phosphorylation in isolated mitochondria so its effect on cell division would not be secondary to the inhibition of energy conservation. It s possible inhibition of enzyme activity is selective since it also failed to affect RNase activity in vitro.