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Studies on the Germination of Pine Pollen (Pinus mugo) in vitro . I. Growth Conditions and Effects of pH and Temperature on Germination, Tube Growth and Respiration
Author(s) -
Nygaard Per
Publication year - 1969
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1969.tb07384.x
Subject(s) - germination , pollen tube , respiration , pollen , botany , tube (container) , biology , in vitro , pinus <genus> , horticulture , biochemistry , materials science , pollination , composite material
The ability of pine pollen to grow in vitro is discussed in relation to its in situ . Optimal conditions of growth in vitro were investigated. All the experiments were made with pollen of Pinus mugo Turra. A satisfactory medium is described as that which at pH 5.2 and at 29 °C can lead to a tube growth bigger than in vivo . The initial growth (0–30) hours) in terms of tube growth and respiration is described in detail. It is shown that a correlation exists between tube growth and respiration. A method for estimating the metabolism and growth of the pollen tube, based on the uptake of 32 p‐labelled phosphate, is described. Addition of known stimulators of plant growth and extension does not stimulate tube growth. Temperature studies show that under 20°C germination does not take place.