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Changes in Individual Nucleotides of Developing Soybean Seeds
Author(s) -
Galitz Donald S.,
Howell Robert W.
Publication year - 1968
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1968.tb07361.x
Subject(s) - nucleotide , gtp' , perchloric acid , nad+ kinase , chemistry , biochemistry , chromatography , high performance liquid chromatography , enzyme , organic chemistry , gene
Perchloric acid extracts of soybean seeds were separated by column chromatography into 17 peaks. Thirteen nucleotides were identified in 11 of these peaks (NAD, CMP, CDP, CTP, AMP, ADP, ATP, GMP, GDP, GTP, UMP, UDP, and UTP). Two of the peaks were identified as ascorbic acid and dehydroascorhic acid. Four peaks remain unidentified although one of these appears to be protein. Monophosphates decreased and triphosphates increased as development proceeded. Diphosphates were initially low and showed no statistically significant difference with maturity. The accumulation of triphosphates in late stages of development is discussed in relation to synthetic activities of the developing seeds.