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Enzymatic Maceration of Plant Tissue
Author(s) -
Sato Sitiro
Publication year - 1968
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1968.tb07335.x
Subject(s) - ethylenediaminetetraacetic acid , vicia faba , chemistry , chromatography , pectinase , enzyme , substrate (aquarium) , chlorophyll , maceration (sewage) , botany , biochemistry , biology , inorganic chemistry , organic chemistry , ecology , chelation , materials science , composite material
Tissue cells of Vicia faba were separated from the leaf pieces by the pectinase treatment after which the chlorophyll determination method was employed. The separation proceeded at a constant rate for 3 hours', the maximum rate of separation was at pH 5.3. The optimal pH shifted to 5.5 in the presence of 0.001 M 2Na‐ethylenediaminetetraacetic acid (EDTA), which accelerated the separation at pH levels higher than 5.5. The separation was enhanced by NaCl and MgCL 2 and delayed by CaCl 2 and hypertonicity produced by sugars. Indoleacetic acid (IAA) and naphthaleneacetic acid (NAA) accelerated the separation at physiological concentrations. The rate of separation differed markedly by the age of the leaf and the species which offered the substrate material.