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Isoenzyme Polymorphism in Flowering Plants III Gel Electrophoretic Methods and Applications
Author(s) -
Brewbaker James L.,
Upadhya Mahesh D.,
Mäkinen Yrjö,
Macdonald Timothy
Publication year - 1968
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1968.tb07319.x
Subject(s) - isozyme , biochemistry , peroxidase , electrophoresis , catalase , leucine , polyacrylamide gel electrophoresis , staining , enzyme , biology , chemistry , alkaline phosphatase , amylase , microbiology and biotechnology , amino acid , genetics
Gel electrophoretic methods and equipment which were devised or modified for rapid and economic application to angiosperm tissues are described. Staining schedules are outlined that proved effective on a wide variety of tissues for elucidation of isoenzymes with the following activities; eslerase, peroxidase, catalase, leucine amino peptidase, phosphatase, amylase, and glutamic‐oxalacetic‐transaminase. Illustrative applications are discussed.