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Carbonic Anhydrase in the Marine Alga Ulva pertusa
Author(s) -
Ikemori M.,
Nishida K.
Publication year - 1968
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1968.tb07252.x
Subject(s) - sulfanilamide , carbonic anhydrase , sulfonamide , chemistry , enzyme , cysteine , darkness , biochemistry , botany , chromatography , biology , stereochemistry
The preparation of stable samples containing carbonic anhydrase from Ulva pertusa was achieved by using artificial sea water and 10 m M cysteine solution adjusted to pH 8.2 instead of 10 m M cysteine solution alone. When the sample was stored at 0°C in darkness the enzyme activity was retained for about 24 h. In contrast to the results obtained with land plants in previous works a remarkable inhibitory effect of low concentrations of sulfanilamide and Diamox (2‐acetylamino‐1,3,4‐thiadiazole‐5‐sulfonamide) on the activity of the enzyme was observed.