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Dependence of Lettuce Seed Germination on Actinomycin D‐resistant RNA Synthesis
Author(s) -
Khan Anwar A.
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb08390.x
Subject(s) - gibberellic acid , orotic acid , radicle , cycloheximide , germination , uracil , rna , elongation , biology , secale , nucleic acid , puromycin , protein biosynthesis , kinetin , thiouracil , biochemistry , botany , tissue culture , dna , materials science , ultimate tensile strength , gene , metallurgy , in vitro , thyroid , endocrinology
The response of Grand Rapids lettuce half seed and intact seed to different nucleic acid and protein synthesis inhibitors was studied. 6‐azauracil, 2‐thiouracil and cycloheximide were strong inhibitors of germination of intact seed as well as of radicle elongation of half seed. Inhibition by 6‐azauracil and 2‐thiouracil was reversed by uracil but not by orotic acid, thymine or deoxycytidine. Actinomycin D and puromycin were ineffective suggesting that actinomycin D‐resistant RNA synthesis possibly controls both germination of intact seed and radicle elongation of half seed. The requirements of RNA synthesis for the elongation of radicle in half seed in light and in the presence of gibberellic acid was the same. Such was not the case in the germination of intact seed which had different requirements of RNA synthesis in light and in presence of gibberellic acid. It is concluded that the mechanism of gibberellic acid and light in the intact seed germination, in so far as it involves the regulation of RNA synthesis, depends on the integrity of the seed coats.