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Copper Oxidases in Thalli of the Liverwort Marchantia polymorpha
Author(s) -
Poucke M. Van
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb08381.x
Subject(s) - marchantia polymorpha , thallus , ascorbic acid , enzyme , oxidase test , biochemistry , chemistry , copper , intracellular , biology , botany , organic chemistry , food science , gene
A study is presented on the properties of copper oxidases in thalli of the liverwort Marchantia polymorpha L. Catecholase and ascorbic acid oxidase are compared in their specific activity, reaction kinetics, inhibitor sensitivity and intracellular localization. It is shown that catecholase is predominantly bound to particulate cell fractions and is normally inhibited by the usual inactivators of copper enzymes. Ascorbic acid oxidase activity, on the other hand, is strongest in the soluble protein fraction and is quite resistant toward the same inhibitors at the pH‐optimum of the enzyme. Both enzymes, when assayed with small tissue fragments, can be inhibited as expected.