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Some Characteristics of Rapidly Labeled RNA in Jack Beau Leaves
Author(s) -
Matsumoto H.,
Kobayashi M.,
Takahashi E.
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb08380.x
Subject(s) - rna , ribosomal rna , incubation , nucleotide , microbiology and biotechnology , biochemistry , biology , composition (language) , dna , elution , chromatography , chemistry , gene , philosophy , linguistics
The characteristics of rapidly labeled RNA in jack bean leaves were investigated. The RNA was prepared by the phenol method and fractionated with methylated serum albumin column. The composition of DNA and ribosomal RNA were also determined. The labeled RNA which appeared after 90 minutes' incubation could be differentiated clearly from the ribosomal RNA by means of the elution pattern and the nucleotide composition. The RNA labeled for 90 minutes disappeared completely after 210 minutes' incubation and the other three peaks appeared at almost the same position as ribosomal RNA.