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Cytokinin Activity in Begonia and Bryophyllum
Author(s) -
Heide Ola M.,
Skoog Folke
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb07220.x
Subject(s) - begonia , chromatography , cytokinin , chemistry , bioassay , fractionation , botany , callus , biology , biochemistry , genetics , auxin , gene
Cytokinin activity has been obtained in ethanol extracts of Begonia and Bryophyllum plants. Begonia x cheimantha Everett yielded 30 to 300 μg kinetin equivalents per kg of fresh leaves in the tobacco callus bioassay. Short day conditions appeared to increase the extractable cytokinin content in the tissue. Purification by fractionation on Dowex 50 H + columns followed by organic solvent extraction, silver precipitation, and repeated paper chromatography yielded an apparently homogeneous product which accounted for most of the activity in the Begonia extracts. It was indistinguishable from zeatin in chromatograms developed with six solvent systems. Other cytokinin active fractions were also obtained from both Begonia and Bryophyllum . Crystalline picrate preparations of active products were insufficient for identification by mass spectrometry.