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Subcellular Location of Phosphoglucomutase and UDP Glucose Pyrophosphorylase in Avena Coleoptiles
Author(s) -
Hall M. A.,
Ordin L.
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb07204.x
Subject(s) - phosphoglucomutase , avena , coleoptile , biochemistry , chemistry , enzyme , dehydrogenase , iodoacetamide , chromatography , biology , botany , cysteine
Extraction of phosphoglucomutuse and UDP glucose pyrophosphorylase from oat coleoptiles using both aqueous and non‐aqueous systems showed that both enzymes are largely soluble. Phosphoglucomutase was completely absent from the cell wall fraction. The inhibition of phosphoglucomutase by peroxyacetyl nitrate was confirmed and the enzyme in the subcellular participate fractions was shown to be more inhibited than that in the soluble fraction. UDP glucose pyrophosphorylase was not inhibited by peroxyacetyl nitrate or ozone in vivo but was inhibited by peroxyacetyl nitrate in vitro . The SH reagents, iodoacetamide and p ‐chloromercuribenzoate, inhibited phosphoglucomutase severely but UDP glucose pyrophosphorylase moderately or not at all.