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Growth and Development of Pelargonium Pith Cells in vitro
Author(s) -
Chen HwaRuey,
Galston Arthur W.
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb07194.x
Subject(s) - pith , callus , cytokinin , auxin , pelargonium , explant culture , botany , shoot , biology , in vitro , horticulture , chemistry , biochemistry , gene
Pelargonium pith callus cultured in media containing auxin and cytokinin will not differentiate formed organs if monthly subcultures are made into fresh medium. Left on semisolid medium for prolonged periods, such callus gives rise to nodules containing lignified cells. Such nodules can be stimulated to produce shoots and roots if cultured for one month in shaken liquid medium or for longer times on semisolid mediium lacking both auxin and cytokinin. The subsequent development of organs is best on semisolid medium. Entire plants have heen produced in this way from initial pith explants containing several hundred cells.