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Effects of Ultraviolet and X‐Ray Radiation on in vitro Cultivated Cells of Haplopappus gracilis
Author(s) -
Eriksson Tage
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb07191.x
Subject(s) - chromosome , mitosis , irradiation , x ray , cell , cell division , radiosensitivity , biophysics , biology , ultraviolet , in vitro , mutant , plating efficiency , strain (injury) , microbiology and biotechnology , chemistry , genetics , optics , physics , anatomy , nuclear physics , gene
A cell strain of Haplopappus gracills was used for investigations of the effects of UV (2537 A) and X‐ray irradiation. Mitotic inhibition and killing after UV exposure were studied. A survival curve of UV treated and then plated cells is presented. The LD 50 seems to be about 2000 erg. mm −2 under the experimental conditions used. All types of chromosome aberrations are induced by UV irradiation, but the frequency is relatively low at doses which do not completely inhibit cell division. A mutant strain of chromosome type is isolated after UV treatment and then plating. Mitotic inhibition and killing after X‐ray treatment were studied. A survival curve is presented and the LD 50 under the culture conditions used seems to be about 2000 R. The frequency of chromosome aberrations induced by X‐rays is highly increased by aeration during X‐ray treatment which indicates that some degree of cell anoxia exists in a cell suspension. There arr indications that chromosome aherrations may not cause growth inhibition to such an extent as is usually believed.