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Gibberellin‐induced Yeast Sporulation in Relation to RNA and Protein Metabolism
Author(s) -
Kamisaka Seiichiro,
Masuda Yoshio,
Yanagishima Naohiko
Publication year - 1967
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1967.tb07146.x
Subject(s) - ethionine , puromycin , uracil , thiouracil , guanine , biochemistry , methionine , chloramphenicol , protein biosynthesis , rna , yeast , chemistry , biology , dna , thyroid , amino acid , antibiotics , nucleotide , genetics , gene
Gibberellic acid (GA) promoted sporulation in yeast when added to the sporulation medium. When added together with GA, metabolic inhibitors of RNA synthesis such as 8‐azaguanine, 2‐thiouracil, and actinomycin D suppressed selectively the promoting effect of GA on sporulation. The effect of 8‐azaguanine and 2‐thiouracil was alleviated by simultaneous addition of guanine and uracil, respectively. The promoting effect of GA on sporulation was also suppressed by inhibitors of protein synthesis such as ethionine, chloramphenicol, and puromycin. Methionine eliminated the inhibitory effect of ethionine on the GA action.