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Auxin Activity of Glucobrassicin
Author(s) -
Andersen A. Skytt,
Muir Robert M.
Publication year - 1966
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1966.tb07094.x
Subject(s) - auxin , hypocotyl , glucosinolate , epicotyl , botany , etiolation , biology , chemistry , indole 3 acetic acid , brassica , biochemistry , enzyme , gene
The indole‐glucosinolate, glucobrassicin, which is found in large amounts in members of the genus Brassica , was subjected to tests in the standard Avena curvature bioassay. Both the tetramethylammonium salt of glucobrassicin and the non‐crystallizted material induced curvature at a concentration of 5 × l0 −4 M . The curvature from the salt was, however, only half of that induced by the acid form. Bioassays of chromatograms of fresh methanolic extracts of savoy cabbage indicated the presence of indole‐3‐acetic acid as well as indole‐3‐acetonitrile in small amounts along with large amounts of glucobrassicin. Segments of green hypocotyl tissue of savoy cabbage respond with limited growth to high concentrations of glucobrassicin. Segments of etiolated hypocotyls or green epicotyls give no growth response to glucobrassicin. Pea stem segments which do not respond to indoleacetonitrile responded with similar growth to glucobrassicin and indoleacetic acid. Nitrilase activity was found in savoy hypocotyl and epicotyl tissue by chromatography of incubation media. The growth regulation in savoy cabbage in conjunction with data on the distribution of glucobrassicin in the plant, leads to the conclusion that glucobrassicin is a special, inactive storage and transport form of the active auxin in cabbage.