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Effect of Tobacco Mosaic Virus Infection on Amino Acid Incorporation into Isolated Mitochondria from Tobacco Leaves
Author(s) -
Takahashi Tsuyoshi,
Hirai Tokuzo
Publication year - 1966
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1966.tb07079.x
Subject(s) - tobacco mosaic virus , biochemistry , deoxyribonuclease , mitochondrion , adenosine triphosphate , amino acid , puromycin , guanosine , biology , guanosine triphosphate , cell free system , in vitro , gtp' , protein biosynthesis , microbiology and biotechnology , dna , enzyme , virus , virology
Mitochondria isolated from tobacco leaves incorporated 14 C‐leucine into the protein and the rate was enhanced by tobacco mosaic virus (TMV) infection as compared with noninfected level. In vitro amino acid incorporation by mitochondria required adenosine triphosphate (ATP), Mg 2+ , and KC1 and the energy sources from oxidative phosphorylation as well as from ATP‐generating system. This incorporation was inhibited by ribonuclease (RNase), deoxyribonuclease (DNase), actinomycin D, mitomycin C, puromycin, and chloramphenicol added in the reaction medium. The pretreatment of the mitochondria with DNase and actinomycin D reduced the rate of incorporation. The mitochondria incorporated 3 H‐guanosine triphosphate (GTP) and this activity was blocked by actinomycin D. The presence in this system of 15,000 g supernatant cell sap fraction or bacterial contamination was carefully checked obtaining a negative result. The reaction product into which l4 C‐amino acids incorporated was solubilized by trypsin. The nature of the amino acid incorporating activity of isolated mitochondria obtained from TMV‐infected tobacco leaves is discussed.