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Dietary docosahexaenoic acid in combination with arachidonic acid ameliorates allergen‐induced dermatitis in mice
Author(s) -
Weise Christin,
Heunemann Carolin,
Loddenkemper Christoph,
Herz Udo,
van Tol Eric A.F.,
Worm Margitta
Publication year - 2011
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.2010.01133.x
Subject(s) - docosahexaenoic acid , medicine , arachidonic acid , thymic stromal lymphopoietin , cytokine , ovalbumin , atopic dermatitis , immunology , allergy , allergen , allergic inflammation , proinflammatory cytokine , inflammation , polyunsaturated fatty acid , fatty acid , biochemistry , biology , immune system , enzyme
To cite this article: Weise C, Heunemann C, Loddenkemper C, Herz U, van Tol EAF, Worm M. Dietary docosahexaenoic acid in combination with arachidonic acid ameliorates allergen‐induced dermatitis in mice. Pediatr Allergy Immunol 2011; 22 : 497–504. Abstract Objective: In this study, we investigated the impact of dietary docosahexaenoic (DHA) and arachidonic acid (AA) on development and severity of allergen‐induced dermatitis. Study design: In sensitized mice, skin inflammation was induced by ovalbumin. Mice received either a diet containing 0.015% DHA, 0.029% AA or the combination of both. The severity of dermatitis was evaluated by using a clinical skin score (CSS), followed by immunohistologic and cytokine analysis. To unravel potential mechanisms, interleukin (IL)‐4 or tumor necrosis factor α–stimulated keratinocytes from the cell line Kera‐308 was cultured with different DHA/AA compositions and analyzed regarding proliferation and cytokine production. Results: Dietary DHA/AA significantly improved the severity of allergen‐induced dermatitis as the CSS was reduced by 36 ± 23% (p = 0.005). Furthermore, reduced epidermal KI67 expression, increased number of forkhead box P3 + cells, and elevated IL‐10 expression were determined in skin lesions of dietary‐treated mice. Correspondingly, in vitro DHA/AA‐treated keratinocytes exhibited increased IL‐10 expression and produced less thymic stromal lymphopoietin. Conclusion: Dietary DHA/AA supplementation leads to a significant amelioration of allergen‐induced dermatitis. This was accompanied with the presence of increased regulatory T cells and IL‐10 expression in lesional skin. Moreover, we identify keratinocytes, which play a crucial role in the regulation of skin inflammation, as important targets of DHA/AA supplementation. Future studies are needed to clarify whether DHA/AA acts directly or whether its biologic active metabolites are responsible for these findings. This may unravel novel therapeutical compounds for allergen‐induced dermatitis.